Calibration curve - including information from replicates Kuba  2021-03-02 02:26
 

Hi Skyline Support,

I have a question related to how the calibration curve is presented.

I am trying to create a calibration curve for my heavy peptides at different injected concentrations. The injections were done in triplicates for each concentration of the peptide.

At the moment I can see calibration curves with concentration data points individually for each replicate. What I would like to see instead is a single point as the average of the three runs and the standard deviation. Is it possible in skyline? or do I need to export peak intensities for each peptide concentration individually and plot that externally?

Many thanks

Kuba
 
 
Nick Shulman responded:  2021-03-02 09:41
There is no way to make the Calibration curve in Skyline display the points the way that you want.

If you want to export the mean and standard deviations of the peak areas you should use the Pivot Editor in the Document Grid.

There is a PDF which walks you through how to use the Pivot Editor to calculate the CV of areas across groups of replicates.
https://skyline.ms/wiki/home/software/Skyline/page.view?name=PivotEditor

You will probably be able to figure out how to adapt those steps in order to get Skyline to calculate the mean and standard deviation of the areas across the replicates which have the same Analyte Concentration.
Let me know if you get stuck.
-- Nick