Display of protein abundances in PRM experiment | bpfister | 2021-02-05 02:55 | |||||||||||||||||||||
Dear Skyline team, I was very excited about the recent implementation to show the actual protein abundances of each replicate within the Group Comparison grid, but am having problems with displaying them correctly. As my experiment included spiked-in heavy labelled peptides, I used "ratio to heavy" as the normalization method for the group comparison (see screenshot). I understood that I should also apply this normalization method in Peptide Settings --> Quantification (screenshot), but when I do that, it will only display me N/A for all replicates and proteins in the Group Comparison grid (why?). I am using Skyline version 20.2.0.343. Thanks a lot for your help! |
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