Precursor metabolite data from SWATH | lefelit | 2020-04-09 09:02 | |||||||||||||||||||||||
Hi I have metabolite data from TOF MS acquisition on a SCIEX triple TOF instrument and I am trying to extract the ion chromatogram information for more than 200 precursors. I am not interested at this stage for fragment data. I built a method with all the molecules of interest with the accurate masses and when i applied it to a test mixture of pure standards the software does not find almost 60% of them. I set the mass window to 0.2 and the RT window to 1min and still does not find them. What could be the source of the problem? How could I also customise the threshold setting to different value per precursor? Many thanks |
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