How to caculate the Precision,Extraction recovery or Matrix effect with skyline? | kaylee xu | 2020-02-11 21:08 | |||||||||||||||
Dear skyline team, Q1: Recently, I am using Skyline for small molecular quantitative analysis. At methodological validation, I don't know how to calculated the Precision, Extraction recovery or Matrix effect with Skyline? Can you give me some steps? Q2: If I build 10 compounds' calibrations at a time with different concentrations, eg. Compound-1( 1, 2, 5, 10, 20, 50, 100ng/mL),compound-2(2,4,10,20,40,100,200ng/mL) et.al. How to enter the concentration of these different compounds in the skyline? Q3.What does these black error bar mean? Please see attach file. Q4:Can I quantity one analyte in unknow samples with other analyte's standard curve line ? For example, I want quantity SM18:1 in unknown samples with SM18:1(d9)'s standard curve line. Thank You! Kaylee |
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