Not able to see libray ion in the result file and how to do the quantitation of the peaks

support
Not able to see libray ion in the result file and how to do the quantitation of the peaks drrenugoel  2019-08-23 07:21
 

Kindly see the attached file. I have built the library in the peptide setting tab. However, I am not able to see the library ions in the attached file (skyline_doubt). some setting has been changed as I used to get it. Kindly see the attached (skyline2).

I have run these samples on 5600 sciex in MRM-HR mode and now want to do the quantitation. Could you please let me know how to take it out peak area for the same.

 
 
Brendan MacLean responded:  2019-08-23 07:51

You should be able to switch back with View (or right-click) > Transitions > All. It appears you are currently in View > Transitions > Total mode.

Thanks for posting to the support board and supplying the screenshots.

--Brendan

 
drrenugoel responded:  2019-08-23 08:06

Thank you so much. fantastic. I had run 5 biological replicates from three conditions. I would like to do the quantitation. Could you please help?

 
Brendan MacLean responded:  2019-08-24 21:28

This is probably a good Webinar for you:

https://skyline.ms/webinar08.url

And this tutorial:

https://skyline.ms/tutorial_grouped.url