Kindly see the attached file. I have built the library in the peptide setting tab. However, I am not able to see the library ions in the attached file (skyline_doubt). some setting has been changed as I used to get it. Kindly see the attached (skyline2).
I have run these samples on 5600 sciex in MRM-HR mode and now want to do the quantitation. Could you please let me know how to take it out peak area for the same.