Issue importing waters RAW files, Unhandled Exception: Invalid Function Type Allison Haase  2019-08-19 08:53
 

Hi All,

I have been getting error messages in skylines when importing raw files from a specific set of experiments on a Waters XEVO. Other files that have been imported from this instrument have worked fine in the past, and the raw files can be opened in MassLynx. The error is pasted below.

At 9:45 AM:
Failed importing results file 'C:\Users\allis\Desktop\redownload\20190809r001.raw'.
[pwiz::CLI::msdata::ChromatogramList::size] Unhandled exception: Invalid Function Type

Inner exceptions:
Exception type: System.Exception
Error message: [pwiz::CLI::msdata::ChromatogramList::size] Unhandled exception: Invalid Function Type
[pwiz::CLI::msdata::ChromatogramList::size] Unhandled exception: Invalid Function Type
at pwiz.CLI.msdata.ChromatogramList.size()
at pwiz.ProteowizardWrapper.MsDataFileImpl.get_HasChromatogramData() in C:\proj\skyline_19_1_x64\pwiz_tools\Shared\ProteowizardWrapper\MsDataFileImpl.cs:line 844
at pwiz.Skyline.Model.Results.ChromatogramDataProvider.HasChromatogramData(MsDataFileImpl dataFile) in C:\proj\skyline_19_1_x64\pwiz_tools\Skyline\Model\Results\ChromDataProvider.cs:line 365
at pwiz.Skyline.Model.Results.ChromCacheBuilder.BuildCache() in C:\proj\skyline_19_1_x64\pwiz_tools\Skyline\Model\Results\ChromCacheBuilder.cs:line 194

I have also attached one of the raw files in a zip folder.

Thank you very much for your time,
Allison

 
 
Matt Chambers responded:  2019-08-22 10:19

Hi Allison,

Thanks for the RAW file to test with. I can reproduce the issue and I have forwarded it to our Waters contact for troubleshooting.

 
haihaba responded:  2020-02-05 13:05

Hi Matt,

Do you have any progress of this thread?

I have the same issue.

At 3:45 PM:
Failed importing results file 'M:\Users\Imhoi.Koo\Data\inPSU\Yuan\TY_Upenn fecal_190326_0006.raw\TY_Upenn fecal_190326_0006.raw'.
[pwiz::CLI::msdata::ChromatogramList::size] Unhandled exception: Invalid Function Type


Inner exceptions:
Exception type: System.Exception
Error message: [pwiz::CLI::msdata::ChromatogramList::size] Unhandled exception: Invalid Function Type
[pwiz::CLI::msdata::ChromatogramList::size] Unhandled exception: Invalid Function Type
   at pwiz.CLI.msdata.ChromatogramList.size()
   at pwiz.ProteowizardWrapper.MsDataFileImpl.get_HasChromatogramData() in C:\proj\skyline_20_1_x64\pwiz_tools\Shared\ProteowizardWrapper\MsDataFileImpl.cs:line 957
   at pwiz.Skyline.Model.Results.ChromatogramDataProvider.HasChromatogramData(MsDataFileImpl dataFile) in C:\proj\skyline_20_1_x64\pwiz_tools\Skyline\Model\Results\ChromDataProvider.cs:line 461
   at pwiz.Skyline.Model.Results.ChromCacheBuilder.BuildCache() in C:\proj\skyline_20_1_x64\pwiz_tools\Skyline\Model\Results\ChromCacheBuilder.cs:line 231

I tried to convert my data into mzML, but it was failed.

I attached one of my file.

Please look at it and if you have any solution please let me know.

Thanks,

Imhoi

 
Matt Chambers responded:  2021-10-29 12:47

Hi Imhoi,

Sorry it's been such a long time since I got back to looking at this support request. I think this issue is fixed. Could you try again with the latest Skyline-daily?

Thanks,
-Matt