Feature Request Sequence Dependent Precursor Charges | roman sakson | 2019-06-13 05:58 | |||||||||||||||||||||||||||||||||||||||
Hello everyone, first of all, thanks again for the amazing piece of software that continues to be the highlight of my research days! I work in a core facility, where we routinely develop MRM assays from crude synthetic standards without much prior knowledge, this means that for the first runs we have to "guess" the precursor charge state as well as suitable transitions based on common MS sense for relatively long target lists. In our experience, doubly charged precursor ions work best for tryptic peptides of average length, therefore this is the standard transition setting. However, if the sequence contains histidine, normally the more intensive precursor is triply charged. Is it possible to specify in the filter settings or elsewhere, that precursors should be doubly charged, unless there is a particular amino acid present in the sequence? Thanks a lot in advance for your time! Roman (Heidelberg, Germany) |
|||||||||||||||||||||||||||||||||||||||||
| |||||||||||||||||||||||||||||||||||||||||