System.IO.IOException: ERROR: Illegal character p found in sequence ..... | jason a macgurn | 2019-04-15 14:28 | |||||||||||||||||||||||||||||||||||
Hello, I am using skyline with MS1 full scan filtering on data acquired from a Q Exactive and searched on MaxQuant. I am using Skyline to inspect peptides (especially phosphopeptides) and validate quantification from SILAC labeling. I had been using a very nice workflow (based on the MS1 full scan filtering tutorial) that was perfect for our application. But recently, I had some computer problems which forced me to install the latest versions of both MaxQuant and Skyline, and now whenever I try to set up the filtering on my data I receive the following error: "ERROR: Illegal character p found in sequence AAGpSGESpTPER (line 13)" I only get this error when I attempt to filter data generated with my new version of MaxQuant - if I filter analysis from the old version, there are no errors. My interpretation is that Skyline doesn't like something about the way MaxQuant is formatting the msms.txt output file (phosphopeptides specifically?), so it chokes on it. I figure I either need to re-format the msms.txt output file or adjust some setting for the input on Skyline. Has anyone else encountered this problem? Is there a fix or workaround? |
|||||||||||||||||||||||||||||||||||||
| |||||||||||||||||||||||||||||||||||||