Error Line 705 at column 9 Kendra Adams  2019-02-06 09:59
 

Hi Skyline Team,
I am having an issue with a document I just created in Skyline Daily. Seemed to work fine while I had it open, and then fails after I close and try to open it again.
The error message is attached.
Thanks
Kendra

 
 
Brian Pratt responded:  2019-02-06 10:44

Can you provide the actual document so we can investigate?

Thanks

Brian Pratt

 
Kendra Adams responded:  2019-02-07 05:47

Hi Brian,
Thanks for the quick reply! I made a new document yesterday and I'm getting the error again, unfortunately I cannot zip the skyline document since it will not open, I've attached the new error message and the individual skyline files, let me know if I need to do anything else.
Thanks!
Kendra

 
Brian Pratt responded:  2019-02-07 14:07

Hi Kendra,

We're working on this, but for now here's a workaround:

Open the .sky file in any text editor and find the line containing
precursor_res="120000"
and change that to
precursor_res="60000"

This changes the Full Scan MS1 filtering Resolving power, which changes Skyline's idea of which calculated precursor isotopes are viable. Skyline is applying a test on file read that it didn't seem to have applied when the file was being created, and one or more M+1 isotopes are being rejected as being inconsistent with the settings. We're looking into that, but it comes down to we should always read what we write.

Cheers

Brian