Lipidomic Analysis | elizabeth waldron | 2018-02-08 01:27 | |||||||||
I analysed labelled lipid standards using a DDA experiment on the Q-Exactive machine. I imported my data into Skyline and found that Skyline was only extracting data around a specific time frame. The outcome is that my MS1 data is being extracted beautifully, but my MS2 data looks like a scheduling experiment gone wrong. I have attached a screenshot of my Skyline document. Can anyone advise why Skyline is extracting MS2 data in a limited time frame and not over the whole chromatogram? |
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