Dear Skyline Users,

I have just released a patch to Skyline 21.1 which took longer than usual in an effort to respond to confusion caused by our DIA tutorials and Import Peptide Search wizard discovered in working with the authors of a new DIA comparison paper (https://pubmed.ncbi.nlm.nih.gov/34472865/). Thanks to the authors for contacting us and helping us to understand the initial confusion.

We think the result will benefit the entire Skyline community, and we encourage you to review this paper and the revised tutorials.

This release contains the following improvements over the initial 21.1 release:

• Finalized Chinese and Japanese translation text
• Fixes to Import Peptide Search wizard for DIA tutorial and DIA/SWATH tutorial updates.
• Fixes for DIA-Umpire integration (various).
• Fixed an unexpected error with Ion Mobility settings.
• Fixed an unexpected error with the Full-Scan graph showing when displayed peptide is deleted.
• Fixed an unexpected error in the heatmap graph.
• Fixed a problem introduced in 21.1 where transition list columns were not identified as numeric if the values were in quotes. (impacting LipidCreator)
• Fixed Import Peptide Search FASTA page to hide panel for importing targets from a separate FASTA when not doing a DDA/DIA search.
• Fixed a missing DLL issue with Percolator in MS Amanda DDA search.
• Fixed storing calculated CE and DP values in .sky files with large target lists for Panorama.

We think these improvements make Skyline 21.1 even better and hope you will update to this latest version soon.

Skyline should ask you to update the next time you restart or use Help > Check for Updates.

Enjoy.

--Brendan

P.S. - Remember to register for the Skyline User Group Meeting