I have been using Skyline for several months now and find it an invaluable resource. Its functionality means that it easily surpasses all competitors in terms of ease of use, meaning that time can be spent performing meaningful experiments rather than laboriously building acquisition methods. The regular updates and improvements to the program means that it is becoming increasingly useful in the area of quantitative proteomics using SRM, and thus will continue to be the number one software in this field.

When I had a problem with Skyline, Brendan Maclean (the program's designer) was more than happy to work on altering the program to meet my needs, and did so in a timely manner. The implemented change has improved Skyline in the context of my work, and I am very grateful to Brendan for his help.

Stephen W. Holman Ph.D
Michael Barber Centre for Mass Spectrometry
Manchester Interdisciplinary Biocentre
The University of Manchester
Manchester
M1 7DN
UK